What actually happens.
Two phases: the route into treatment, then what is happening in the body, and inside the laboratory, at every step. In plain English.

The route into treatment.
Most private clinics run the same sequence: a paid first consultation, then the clinic's own blood tests, then a treatment plan. Between the consultation (usually £150 to £300) and the tests (£200 to £500), many people spend around £500 before treatment has started. Some of that is optional. Here is the usual route, and where you have a choice.
In short: the doctor's assessment, infection screening and consent are required. Paying the clinic for every diagnostic test is not. Check a clinic's policy on outside results before you book.
Start with your GP
Your choiceYour GP can arrange the first fertility tests on the NHS for free: blood tests to check ovulation and hormone levels, a chlamydia test and, for a partner, a semen analysis. They can also refer you for NHS treatment if you are eligible.
The NHS suggests seeing your GP after a year of trying, or sooner if you are 36 or over or have a known concern. If you are single or in a same-sex couple, you do not need to have been trying first.
Ask: Can I have printed copies of every result? They are yours, and any clinic can use them.
Get your baseline tests where you choose
Your choiceBefore IVF a clinic will want to see your ovarian reserve (an AMH blood test and an antral follicle count scan) and, if sperm from a partner is being used, a semen analysis. You do not have to buy these from the clinic. Your GP, an independent lab or a mail-in home kit can often give you results faster and for less.
Clinics do not have to accept outside results, though, and many put a shelf life on them. Blood tests such as thyroid and the infection screen often have to be from the last 3 months. AMH may be accepted for longer, but some clinics repeat it because labs use different methods, and some will not accept finger-prick home samples.
Ask: Before you pay for a test: do you accept outside results, from which labs, and how recent must each one be? Then time your bloods so they are still in date when you book.
Testing at home
Your choiceA mail-in kit lets you take a finger-prick blood sample or a semen sample at home and post it to a lab. Most check AMH and other hormones, or sperm count and movement. They can be a quick, private first look, and a way to have results in hand before your first appointment.
Know what they can and cannot tell you. The HFEA says hormone tests like these give some indication of fertility, but the results are not guaranteed, and AMH does not predict your chance of getting pregnant naturally. A home sperm test usually measures only count and movement, so a normal result does not rule out a problem, and a low one should be repeated by a lab.
If you buy one, choose a kit marked UKCA or CE for home use, sold by a pharmacy or provider you can check, that sends samples to an accredited lab (UKAS, ISO 15189) and gives you a written report a clinic can read. We do not take payment from test providers or recommend a brand.
Ask: Will you accept a home kit result, and from which labs? If not, the test can still help you decide what to do next, but budget for the clinic to repeat it.
Book a first consultation, with your results in hand
RequiredA licensed clinic has to assess you before it can treat you, so a first appointment with a doctor cannot be skipped. You can make it count. Send your test results and history (how long you have been trying, past tests, past treatment) ahead of time, so the appointment is spent on a plan rather than on paperwork.
Ask: Does the fee come off my treatment? Is there a free open evening or nurse call I can use first?
Infection screening
RequiredBy law, anyone whose eggs, sperm or embryos will be used or stored must be screened for HIV and hepatitis B and C (and sometimes HTLV) before treatment. It is usually done within three months of starting. Most clinics run it themselves, so ask how they handle it and what it costs.
Get a written, costed plan
Your rightConsumer law guidance from the Competition and Markets Authority says clinics must give you clear prices before you commit, including what the headline price leaves out: drugs, scans, freezing, storage and any add-ons.
Ask: Can I have this in writing, with everything the headline price leaves out? Then compare it with another clinic: your test results travel with you.
Consent and counselling
RequiredYou will sign HFEA consent forms covering how your eggs, sperm and embryos are used and stored. Licensed clinics must offer you counselling before you consent. Then treatment begins with Step 01.
Ovarian Stimulation
In a natural cycle, one follicle matures and releases one egg. Stimulation changes that. Daily hormone injections, usually FSH (follicle-stimulating hormone), encourage both ovaries to develop multiple follicles at the same time.
During this phase you will attend monitoring scans every 2–3 days and have blood tests to track hormone levels. The aim is usually 8–15 mature follicles, though every ovary is different and fewer is not a failure.
- ✓Stimulation typically lasts 10–14 days
- ✓Follicles need to reach roughly 18 mm before retrieval
- ✓Your ovaries may feel heavy or tender; this is expected and normal
- ✓Call your clinic straight away if you have severe bloating, tummy pain or sickness. These can be signs of ovarian hyperstimulation syndrome (OHSS). Call 999 if you have difficulty breathing or chest pain
Egg Retrieval
Around 36 hours after your trigger injection, egg retrieval takes place. Under sedation, an ultrasound probe with a very fine attached needle is passed transvaginally to reach each follicle. The needle punctures the follicle wall and the fluid inside, including the egg, is gently aspirated.
An embryologist in the adjacent lab immediately examines the fluid under a microscope to identify and count mature eggs. You'll have sedation (or sometimes a general anaesthetic), so most people feel little or nothing, and most go home within a few hours.
- ✓Most people feel bloated or crampy afterwards but recover the same day
- ✓Mature eggs (MII stage) are the ones that can be fertilised; not all retrieved eggs will be mature
- ✓A retrieval of 6–15 eggs is considered a good response for most patients
Fertilisation in the lab (IVF or ICSI)
The eggs and sperm are brought together in the laboratory in one of two ways. With conventional IVF, each egg is placed in a dish with prepared sperm and left for fertilisation to happen. With ICSI (intracytoplasmic sperm injection), the embryologist selects a single sperm under high magnification and injects it directly into the egg using a microscopic needle.
ICSI is used when sperm quality is a concern: a very low count, poor movement or shape, sperm that had to be surgically retrieved, frozen sperm of lower quality, or a previous cycle in which few or no eggs fertilised. Otherwise conventional IVF is used. The HFEA says success rates for the two are very similar.
- ✓With ICSI, each mature egg is injected with one sperm
- ✓Ask your clinic which method it recommends for you, and why
Fertilisation Confirmed
The morning after insemination or ICSI, the embryologist checks each egg under the microscope. A successfully fertilised egg, now called a zygote, shows two small spheres called pronuclei: one from the egg, one from the sperm.
Not every egg will fertilise. A typical fertilisation rate is 60–80% of mature eggs. Those that don't fertilise are not used further. You will usually receive an update from your clinic on day 1.
- ✓Two pronuclei (2PN) is the sign of normal, successful fertilisation
Cell Division
A fertilised egg begins to divide. By day 2 it has 2–4 cells. By day 3, a typical embryo has 6–8 cells, known as blastomeres. The embryologist grades embryos at each check: cell number, whether the cells are even in size, and how much fragmentation is present.
The cells don't grow: the same cytoplasm from the original egg is simply divided into smaller and smaller units.
- ✓Some embryos develop more slowly at day 3 but catch up by day 5, so don't read too much into early grades
- ✓Most clinics culture embryos to day 5 before selecting one for transfer or freezing
The Blastocyst
By day 5, a good-quality embryo has transformed into a blastocyst: a fluid-filled sphere with two distinct cell populations. The outer layer (trophectoderm) will become the placenta. The inner cell mass (ICM), a tight cluster on one side, will become the baby.
The blastocyst also begins to hatch out of its outer shell (the zona pellucida) in preparation for implantation.
- ✓Roughly half of fertilised eggs reach blastocyst stage (laboratory benchmarks for patients under 40)
- ✓Good expansion and high ICM/TE grades predict better implantation rates
- ✓Blastocysts can be vitrified (frozen) and stored for months or years without significant quality loss
Embryo Transfer
On transfer day, the chosen embryo is loaded into a thin, flexible catheter. You lie as you would for a smear test while the catheter is passed gently through the cervix and into the uterine cavity. The embryo is deposited with a tiny amount of fluid.
Ultrasound guidance is usually used so the team can see exactly where in the uterus the embryo is placed. Most people describe the procedure as no more uncomfortable than a cervical smear. You can drive home and resume normal activity the same day.
- ✓Most clinics now transfer a single embryo to minimise the risk of twins
- ✓Bed rest after transfer is not recommended; normal gentle activity is fine
- ✓Remaining good-quality embryos are vitrified and stored for future frozen embryo transfer (FET) cycles
Implantation
After transfer, the blastocyst floats free in the uterus for a day or two before making contact with the endometrium, the uterine lining. The trophectoderm cells begin to burrow into the lining in a process called implantation. Blood vessels start to form between embryo and uterus.
As the embryo embeds, it starts producing hCG (human chorionic gonadotropin), the hormone detected by pregnancy tests. Your clinic will give you a test date, usually about two weeks after transfer.
- ✓Implantation typically occurs 6–10 days after fertilisation
- ✓A beta-hCG blood test is more sensitive and accurate than a home pregnancy test
- ✓Even a perfectly graded blastocyst doesn't always implant; chromosomal abnormalities in the embryo are the most common reason for failure
In 2024 the UK average birth rate per embryo transferred was 30%: 38% for patients aged 18 to 34 and 8% at 43 to 44 (HFEA, preliminary figures).
Mental health: counselling and support.
Sources
- HFEA: Fertility treatment 2024, trends and figures
- HFEA: In vitro fertilisation (IVF)
- NHS: IVF, what happens
- HFEA: Intracytoplasmic sperm injection (ICSI)
- NICE: Fertility problems, assessment and management (NG257)
- NHS: Infertility, diagnosis
- HFEA: Single women (fertility MOTs)
- Manchester University NHS Foundation Trust: Fertility pathway guide (AMH)
- Evaluation of at-home serum AMH testing: a head-to-head comparison study
- Review: at-home semen testing and its limitations
- CMA: Fertility clinics, complying with consumer law
- ESHRE/Alpha Vienna consensus on laboratory performance indicators (fertilisation and blastocyst rates)
Clinical content last reviewed October 2026. Figures like fertilisation and blastocyst rates are laboratory averages, not predictions for any one cycle, and protocols vary between clinics. Nothing on this page is medical advice: your clinic’s guidance about your own treatment always takes precedence.